动物造模,小鼠炎症模型 z-bio 2021-01-15 367

　　Objective: To analyze the pathogenicity of influenza A virus using mouse inflammation animal model.

　　Method: Three different types of influenza A/pig/Jiangsu/C1/08(H9N2)(H9C1)? Is it /pig/Shandong/731/2009 (SD731)? A/PutoRico/8/34 (H1N1) (PR8), a 5-week-old BALB/c mouse was nasally infected with 1×106 TCID50, clinical symptoms were observed, and the viral load in the lung tissue was detected with inflammation. What is Gender factor? "

　　Result: In the pathogenicity experiment of BALB/c mice, the lung tissue inflammation and virus replication levels caused by SD731 and PR8 viruses were significantly higher than that of H9C1 and SD731. The mortality rate was 80%, and PR8 was 100. %; H9C1 virus infection is accompanied by weight loss, but does the mouse die?

　　Conclusion: Have you successfully established an inflammation model of mouse influenza virus infection?