动物造模,小鼠乳房炎模型,金黄色葡萄球菌 z-bio 2021-01-15 709

　　Objective: To study the changes of inflammatory factors interleukin 2 (IL-2) and interleukin 4 (IL-4) in the etiology of murine mastitis. Method: 12 lactating female mice (BALB/C), random control group (group A), high concentration group (group B), medium concentration group (group C), low concentration group (group D), it can be divided into . Among them, group A was injected with saline; group B was injected with saline. C? D Inject different concentrations of 1.2 x 105, 1.2 x 104, 6.0 x 103 CFU/mL Staphylococcus aureus. H&E staining to observe pathological changes, qRT-PCR, immunohistochemistry and Westernblot to detect mouse breast tissue.

　　 Results: Does the H&E staining result show that as the concentration of Staphylococcus aureus increases, the pathological changes in breast tissue gradually deteriorate? Compare group B with group A through immunohistochemistry, qRT-PCR and Westernblot methods? C? The expression level of IL-2 in D increased significantly (P\u003c0.05), but with the increase in the concentration of infected bacteria, does the expression level of IL-2 gradually decrease? At the same time, compared with group A. B? C? The expression level of IL-4 in group D increased significantly (P\u003c0.05), but with the increase in the concentration of infectious bacteria, does the expression level of IL-4 gradually increase?

　　 Conclusion: The inflammatory factor IL-2? The development of murine mastitis caused by IL-4 induced by Staphylococcus aureus laid the foundation for the pathological mechanism of Staphylococcus aureus mastitis, and provided scientific and theoretical basis for studying the immune mechanism of breast cancer.