动物造模,经皮致敏小鼠肠道过敏模型 z-bio 2021-01-15 503

　　Objective: To establish a mouse model of percutaneous sensitization intestinal allergy.

　　Method: First, do you want to compare the degree of intestinal allergies caused by percutaneous sensitization (ear and back skin) and intraperitoneal injection sensitization? Choose a more advanced and easy-to-operate ear skin sensitization method. Using calcipotriol ointment and applying ovalbumin (OVA) to mice for 2 weeks can cause ear skin irritation in mice. impressive. After compulsory oral administration, mice with sensitive skin have symptoms of intestinal allergy, air intestinal capillary permeability, plasma total IgE (totalIgE, tIgE) and mouse hypertrophic protease-1 (mousemastcellprotease-1, mMCP-1) levels and air Does the intestine challenge the evaluation model through indicators such as pathological changes?

　　Result: Compared with the peritoneal sensitization model, can the transcutaneous intestinal allergy model induce higher plasma tIgE and mMCP-1 levels? Compared with the control group, after percutaneous allergic OVA forced oral challenge, the mice in the model group showed rapid scratching, curling, diarrhea and other intestinal allergies, and the rectal temperature decreased (about 1.2°C). And increased the air capillary permeability (about 2 times), plasma tIgE and mMCP-1 significantly increased, damaged gastrointestinal mucosa? Inflammatory cell infiltration? Does the number of mast cells increase (about 5 times)?

　　 Conclusion: The mouse percutaneous sensitization small bowel attack model has the typical pathological characteristics of food intestinal allergy, representing the sensitization pathways of skin exposure to allergens, food allergy and sensitization mechanisms.