动物造模,早期胚胎发育毒性阳性模型 z-bio 2021-01-18 304

　　Purpose: To compare the reproductive and developmental indexes of Sprague Dawley (SD) female rats fed cyclophosphamide (CTX) at different doses and times before mating, fertility and early embryo development. Establish a standardized positive control model for toxicity testing (paragraph I).

　　 Method: 150 SD rats, half of the people and half of the people were randomly divided into vehicle control group, CTX 20 mg/kg group and CTX 100 mg/kg group according to their body weight. There are 50 men and half of the men in each group. Female rats were divided into 5 groups 14 days before mating. Two and one intraperitoneal injections (intraperitoneal and intraperitoneal) of 20 mg/kg and 100 mg/kg CTX were given respectively every day, and the vehicle control group was given the same amount of normal saline through the same route. No administration was given to male rats in each group used for mating. The general condition of SD rats was observed every day, and body weight and food intake were measured twice a week. On the 14th day of pregnancy (GD14, GD0 on the day when sperm or vaginal thrombosis was found), female rats were sacrificed for final inspection and pregnancy calculation. Incidence rate, loss rate before transplantation, transplantation rate, average number of corpus luteum, average number of transplants, loss rate after transplantation, average number of births, birth rate, continuous fetal weight and absorption rate.. Results Compared with each control group, each CTX The weight and food intake of SD rats in the group were significantly reduced (P\u003c0.05 or P\u003c0.01), pregnancy rate, and loss rate before transplantation. There was no statistical difference between the transplantation rate, the average number of corpus luteum and implants. The uterine continuous fetal weight in the CTX 20 mg/kg group was significantly reduced (P\u003c0.01), while the CTX transplantation was 100 mg/kg. The loss rate was significantly increased (P\u003c0.01), the average number of births in the uterus, birth rate and continuous fetal weight were significantly reduced, and the absorption rate was significantly increased (P\u003c0.01). .01).

　　 Conclusion: 14 days before mating with CTX, female SD rats were injected 5 times and 1 time intraperitoneally, the doses were 20 mg/kg and 100 mg/kg, respectively. This allows us to establish a positive model for fertility and early embryo toxicity in SD female rats.