动物造模,2型糖尿病小鼠模型 z-bio 2021-01-18 368

　　Objective: To observe the changes in blood glucose in a mouse model of type 2 diabetes, and to explore the feasibility of blood glucose intervention in blood glucose assessment 2 hours after a meal.

　　Method: Choose healthy C57BL/6 mice, and use high-fat diet and streptozotocin (STZ) to induce type 2 diabetes (T2DM) experimental group and set up normal control group. The two groups of continuous fasting blood glucose and continuous random blood glucose were measured respectively, and the blood glucose law of the mice was analyzed. The blood was collected 2 hours after meal as the evaluation point of blood glucose intervention in T2DM mice. We evaluated the scientific nature of glucose. The postprandial blood glucose was verified by long-term observation of blood glucose fluctuations in diabetic mice 2 hours after a meal. The end point of blood glucose intervention was stable for 2 hours. Results: T2DM mice with continuous fasting blood glucose showed a relatively stable range of blood glucose changes in the fasting 2-3 hours after a meal, while in T2DM mice fasting for 4 hours or longer, the blood glucose change range was relatively stable . Blood glucose continued to decrease significantly, but the normal group was able to slowly increase and keep blood glucose relatively stable. Within 2 hours after meal, the blood glucose variability of T2DM mice showed stable within 2 hours after meal.

　　 End: 2 hours after a meal, blood glucose can stably reflect the blood glucose change law in the T2DM mouse model, and has excellent application value in evaluating blood glucose intervention experiments.