动物造模,酒精性心肌病小鼠模型 z-bio 2021-01-18 321

　　Objective: To establish a mouse model of alcoholic cardiomyopathy by feeding liquid feed, and to provide an excellent animal model for studying the mechanism of alcoholic cardiomyopathy.

　　 Method: 16-week-old C57 mice were fed with alcoholic liquid feed and non-alcoholic control feed (alcohol group n = 21; alcohol-free group n = 9), and the alcohol concentration of alcoholic feed. Gradually increase from 4.8%. Up to 5.4%, fed for 8 weeks, weighed weekly, monitored the mortality rate, and performed echocardiography and pathological examinations after 8 weeks.

　　Result: The alcohol feeding group started to lose weight from the second week and continued until the eighth week. Compared with the control group, the weight was significantly reduced. Meanwhile, during the alcohol feeding period, 7 mice in the alcohol group died (accounting for 33.3%). There were no deaths in this group; the results of echocardiography showed that the front and back walls of the ventricles of the alcohol group were significantly thinner than those of the control group, the ventricular diameter increased, and the excretion rate and short axis shortening rate of the heart showed a significant decrease; compared with the control group, alcohol The heart/body mass index of the smaller mice in the group was significantly increased, and the HE staining results also showed that the heart of the mice in the alcohol group was significantly reduced. To be sure, the number has increased greatly. Conclusion After 8 weeks of alcohol solution, mouse hearts showed typical pathological features of alcoholic cardiomyopathy, including overall enlargement, thinning of the left ventricular wall and decreased cardiac function. Therefore, this method can successfully prepare a mouse model of alcoholic cardiomyopathy.