动物造模,大鼠重度创伤失血性休克模型 z-bio 2022-02-24 342

　　Objective: To establish an improved rat model of severe traumatic shock and to explore the protective effect of anesthesia on the STHS rat model. Methods Forty adult healthy male SD rats were selected and randomly divided into 4 groups. The normal group was anesthetized with bilateral femoral arteries, the left femoral vein was catheterized, the rats were awake or maintained anesthesia, the awake control group (CC, n = 10), the anesthesia control group (AC, n = 10), the trauma shock group, open Combined with anesthesia or awake state, use 50% of the time and quantitatively controlled blood loss to create a rat model of severe traumatic hemorrhagic shock. I will. Traumatic hemorrhagic shock group (CTHS, n = 10), anesthetic traumatic hemorrhagic shock group (ATHS, n = 10). During the experiment, observe the overall physiological condition of each group of rats, and continuously monitor the hemodynamic indicators of experimental rats: mean arterial pressure (MAP), heart rate (HR), maximum left ventricular pressure increase/decrease rate (±dp) /dtmax)), arterial blood gas index: pH, oxygen partial pressure (PaO2), carbon dioxide partial pressure (PaCO2), hemoglobin concentration (ctHb), lactic acid (Lac), blood glucose (Glu), bicarbonate concentration (cHCO3-) , Alkaline residue (BE); at the end of the experiment, damage to the rat heart, liver, lungs and kidney organs was observed under the HE staining microscope.

　　Results: CTHS, ATHS group HR, ±dp/dtmax changed significantly over time (P\u003c0.01), ATHS group HR, ±dp/dtmax showed a downward trend, but CTHS group first decreased (T1), then Increased or stable (T2, T3, T4, T5, T6) state; after the ATHS group suffered a shock, the MAP showed a gradual upward trend, and eventually it was 25%-40. Although it returned to the normal percentage level, the CTHS group experienced It drops rapidly after the compensatory growth period. PH, PaCO2, ctHb, cHCO3- and BE in CTHS and ATHS groups showed a gradual decrease. Except for PH and ctHb, the reduction in CTHS group was significantly greater than that in ATHS group (P\u003c0.01). Glu, Lac and PaO2 showed a gradually increasing trend, and the increase in CTHS group was significantly higher than that in ATHS group (P\u003c0.01). Compared with the CTHS group, the liver, heart, lung and kidney damage in the ATHS group was significantly reduced. Conclusion Anesthesia can help protect rats from severe hemorrhagic shock, improve hemodynamics, reduce the accumulation of respiration and metabolites, and reduce damage to various organs.