动物造模,大鼠非酒精性脂肪肝病模型 z-bio 2021-01-18 551

　　Objective: To establish a new type of hyperlipidemia susceptibility (WSHc) rat model of non-alcoholic fatty liver disease, observe the changes in blood biochemical indicators and pathological characteristics, and apply it. And explore the value of scientific research. This is an animal model of NAFLD disease.

　　Method: Twenty 7-8 weeks old WSHc rats were randomly divided into two groups and fed with a normal diet and a high-fat diet. Another 20 Wistar rats of the same age were harvested and treated in the same way. Strain control. After 12 weeks of continuous feeding, the serum levels of TC, TG, ALT and AST were detected, and elastic ultrasound imaging was performed. After the animals were sacrificed, the liver was harvested for HE, Oil Red O, Masson and immunofluorescence staining, and the liver morphology, lipid deposition, inflammation and fibrosis of each group were observed. The liver of WSHc rats after a high-fat diet was analyzed. Characteristics of the lesion. Results Compared with Wistar rats on a high-fat diet, WSHc rats were induced by high fat, and serum total cholesterol was significantly increased, similar to clinical hyperlipidemia patients; liver lipids were abnormal. Deposited in blisters and forming blister fat droplets: Inflammatory cells penetrate around the fat droplets to form inflammatory foci, and there are more macrophages; scattered fibrous lesions can be seen. However, these pathological changes were not seen in the liver tissue of Wistar rats induced by high fat. Conclusion: Compared with hyperlipidemia rats, the blood lipids and liver enzyme levels of WSHc rats after induction of hyperlipidemia are close to clinical levels, and there are more serious lipid deposition and inflammation in the liver tissues, as well as scattered liver fibrosis . As a new ideal NAFLD animal model.