动物造模,过敏性结膜炎小鼠模型 z-bio 2021-01-18 668

　　Objective: To establish a mouse model of allergic conjunctivitis with different antigens, different excitatory pathways, and different ages, compare their immune response status and screen the best sensitization conditions. Establish and provide new ideas and methods.

　　Methods: 65 SPF Balb/c mice, different antigens (eg eggs, ragweed pollen, house dust), different excitatory routes (gavage, spray, subcutaneous injection), different mouse ages (1 week, 2 Weeks) Establish a mouse animal model of allergic conjunctivitis (age, 3 weeks, 8 weeks). T-qPCR is used to detect the relative expression levels of CCL5 mRNA and IL-17 mRNA in mouse conjunctival tissues, and flow cytometry is used to use IL in the spleen single cell suspension against CD4 + T cells. -17% detected. Compared with the negative control group, the expression rates of IL-17, CCL5 mRNA and IL-17 mRNA in the Orbmin group, the house dust mite group and the ragweed pollen group all increased, while the weed pollen group was the most. increased. Compared with the negative control group, the ratio of IL-17 and IL-17 mRNA expression in the subcutaneous injection group, forced feeding group, spray inhalation group and positive control group increased, while the expression of CCL5 mRNA in the forced feeding group was higher than the negative Control group. This is similar. The expression level of this group is close to the positive control group. The ratios of IL-17, CCL5 mRNA and IL-17 mRNA expression levels in 1-week and 2-week-old mice were similar, but significantly lower than 3- and 8-week-old mice.

　　Conclusion: Ragweed pollen has better allergy-inducing properties in establishing mouse animal models of allergic conjunctivitis. Subcutaneous injection of stimulating antigens can obtain satisfactory results in the construction of animal models of allergic conjunctivitis in mice. There are no obvious eye symptoms or signs, but the whole body and local areas are sensitive.