动物造模,颅脑损伤后认知障碍大鼠模型 z-bio 2021-11-01 386

　　Objective: To discuss the preparation and evaluation of animal models of cognitive impairment after head injury.

　　Method: Screen 60 healthy male rats (Sprague Dawley, SD), 48 water maze for experiment, and randomly divided into blank control group, model control group and model group. This model was prepared by electronic Cortical Contusion Impact (eCCI) combined with water maze screening to identify rats with cognitive impairment after head injury (cranial brain injury, TBI). Observe the vital signs, nerve and motor functions of rats; Morris water maze experiment; measure the changes of serum MMP-2, MMP-9, SOD, MDA content.

　　Result: Compare with the blank group. The vital signs of the model group were stable and the behavior indicators were different (P\u003c0.05). There is a significant difference between the incubation period of the water maze experiment and the results of the space exploration experiment (P\u003c0.01). Serum MMP-2 2. The content of MMP-9 and MDA increased significantly (P\u003c0.01), and SOD decreased significantly (P\u003c0.01).

　　 Conclusion: This model preparation strategy can better simulate the cognitive dysfunction after head injury, while providing excellent stability, repeatability, objective evaluation and operability.