动物造模,卵巢过度刺激综合征大鼠模型 z-bio 2022-03-17 333

　　Objective: To screen and compare the methods of establishing rat models of ovarian hyperstimulation syndrome, and to provide an excellent animal model for studying the mechanism of OHSS and developing therapeutic drugs.

　　Method: 603-5 weeks old Wistar and SD rats were randomly divided into groups, and different doses of pregnant horse serum (PMSG) and human chorionic gonadotropin (hCG) were injected to establish an OHSS model in a macroscopic manner. Physical examination, organ index measurement, blood progesterone, estrogen and VEGF concentration measurement methods comprehensively evaluated the established model.

　　Result: 3-week-old SD rats showed obvious ovarian hypertrophy, increased vascular permeability and serum VEGF levels. This is a typical OHSS change and is more suitable for establishing an OHSS rat model.

　　Conclusion: Comparison and screening provide a reliable modeling method. It provides a follow-up animal model suitable for OHSS etiology research and drug development.