动物造模,Graves病动物模型 z-bio 2021-01-19 331

　　Purpose: This study starts with the immune-related mechanisms of Graves disease, and compares previous mouse and macaque GD animal models to study the differences in the characteristics of different animal GD models. Based on the characteristics of various animal models, future new methods of immunotherapy will provide research tools.

　　Method: Using recombinant adenovirus expressing adenovirus, BALB/c mice were injected intramuscularly once every three weeks for a total of three times. The red lizard was injected intramuscularly with the same recombinant adenovirus. According to the amount of virus in mice, the amount of virus in red-tailed monkeys is converted to body weight and body surface area once every three weeks, a total of five times. Four weeks after the last immunization, mice and rhesus monkeys were euthanized, and peripheral blood, thyroid, spleen and other tissues were collected to measure total tyrosine TT4, thyroid stimulating hormone receptor antibody TRAb and immune-related indicators. .. Results: The level of TRAb in the mouse model (n = 8) was significantly higher than that in the control group [[(8.1±0.6)IU/Ivs423.1±61.4)IU/I], of which 6 mice had significantly higher TT4 levels high. The total T4 level was significantly higher than that of the control group [(57.1±2.9)μg/dL vs (96.7±13.8)μg/dL, P\u003c0.05], the incidence of GD hyperthyroidism was 75, yes. In the macaque model (n = 6), the TT4 and FT4 levels of the three macaques increased significantly, and the incidence of hyperthyroidism was 50%. In thyroid pathology, mice (6/8) and mackerel (3/6) showed obvious growth of cubic or tall columnar follicular epithelium. Flow cytometry analysis showed that the proportion of Treg cells in the peripheral blood and spleen of the Macaku model was significantly lower than that of the control group (P\u003c0.05), which is the proportion of Treg cells in the spleen of the mouse model. Consistent with the result. (P\u003c0.05). In addition, the modeled Makaku also experienced weight loss (P\u003c0.05) and resting heart rate increase (P\u003c0.05).

　　End: Compared with the Makaku GD model, the mouse model of GD hyperthyroidism has a shorter induction time and a higher incidence, but the basic physiological and biochemical indexes and immune-related indexes of GD Makaku. Shows more similar performance to human GD patients and mechanisms. Future studies to study the etiology of GD and evaluate new treatment options will allow the selection of more appropriate research tools based on the different characteristics of the two animal GD models.