动物造模,子宫内膜异位症纤维化小鼠模型 z-bio 2021-01-19 322

　　Objective: To explore effective methods and evaluation methods for establishing a mouse model of endometriosis fibrosis. Methods: Intraperitoneal injection of BALB/c mouse model of endometriosis fibrosis; collection of mouse peritoneal lavage fluid, determination of TGF-β1 level in lavage fluid in ELISA test kit; mouse abdominal attachment degree Scoring to perform circular diagnosis; HE staining to verify the success of the endometriosis model; Masson staining to detect collagen fibrosis in ectopic lesions and assess the degree of collagen deposition; fibrosis-related protein E-immunohistochemical method, It is used to detect the expression of cadoherin, collagen I and α-SMA, and smooth muscle myosin heavy chain II (SMMHC-II) to assess the degree of ectopic focal fibrosis. Results The success rate of the improved intraperitoneal injection to establish a fibrosis model of mouse endometriosis can reach 100%. The level of TGF-β1 in mouse peritoneal lavage fluid increased significantly with the increase of modeling time. Masson staining and fibrosis-related protein immunohistochemical staining showed that the abdominal cavity of endometriosis mice had a fibrous phenotype.

　　 Conclusion: The improved intraperitoneal injection method shows a high success rate in establishing a fibrosis model of endometriosis in mice. The level of TGF-β1 in peritoneal lavage fluid, Masson staining and fibrosis-related protein E-cadherin, type I collagen, α-SMA, SMMHC-II immunohistochemical staining showed pelvic fibers in mice with endometriosis The degree of transformation can be assessed.