动物造模,兔膝骨关节炎模型 z-bio 2021-11-16 601

　　Objective: To establish and evaluate rabbit knee osteoarthritis model through rabbit cartilage defect.

　　 Method: The animals were randomly divided into three groups according to body weight and sex. Sham operation group, model group and positive control glucosamine group each had 12 animals. After 4 weeks of modeling, intragastric administration was started once a day for 4 consecutive weeks. After the last administration, at the expense of animals, the levels of IL-1β and TNF-α in serum and saliva were detected by ELISA kits, joints and synovium were stained with HE, and articular cartilage was scored with Mackin's. Assess the damage.

　　Result: Compared with the sham operation group, the IL-1β and TNF-α levels in the serum and saliva of the model group were significantly increased. Glucosamine can reduce the levels of IL-1β and TNF-a in serum and saliva. HE staining showed changes and defects in cartilage structure in the model group, inflammatory cell infiltration in the synovium, and cartilage scores were significantly higher than those in the sham operation group. Glucosamine can reduce cartilage defects, reduce synovitis cell infiltration and lower cartilage scores.

　　 Conclusion: Articular cartilage perforation can establish a rabbit knee osteoarthritis model that reflects its main characteristics, and the administration of positive control drugs can reduce injury and inflammation.