动物造模,肝纤维化模型 z-bio 2021-11-03 434

　　Objective: To compare the effects of different doses of carbon tetrachloride on inducing liver fibrosis in irrelevant C57BL/6 mice, and to establish a stable liver fibrosis model.

　　Method: Choose 5 weeks old C57BL/6 irrelevant mice, and inject high, medium and low doses of carbon tetrachloride intraperitoneally to induce liver fibrosis. After the experiment, blood and liver tissue will be collected and plasma measured. The levels and pathological changes of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the liver.

　　Result: After the drug is induced in the dichotomous C57BL/6 mice, the animals in each dose group can survive up to 8 weeks. The survival rate of the high-dose group is only 20%, which is much lower than that of the middle and low-dose groups. .. The pathological changes of liver tissue are related to the influence of dose and time. Pathological analysis showed that hepatocyte degeneration/necrosis, mixed cell infiltration around the central vein/portal vein area and liver fibrosis were observed in each dose group.

　　 Conclusion: 10% carbon tetrachloride administration will induce allogeneic C57BL/6 mice to form a stable liver fibrosis mouse model after 8 weeks, which can be used for follow-up studies of liver fibrosis mechanism and drug screening.