CRISPR/Cas9系统,动物实验 z-bio 2021-11-01 339

　　The team of Professor Jing Yuan from the School of Life Sciences of Xiamen University used the CRISPR/Cas9 system to modify multiple genes of different types in the Plasmodium genome, including gene deletion, gene marking and allele substitution. Related articles were published in "mBio" magazine. Malaria is an infectious disease caused by Plasmodium parasite infection, and it is still a serious public health burden on a global scale. The Plasmodium genome has been sequenced, but the function of most of the genes in this genome is unclear. The original methods of editing and modifying the Plasmodium genome are extremely inefficient, and severely limit the discovery of Plasmodium gene functions.

　　In this research, the team of Professor Yuan Jing from the School of Life Sciences of Xiamen University introduced a DNA double-strand break at a specific site into the genome of Plasmodium yoelii based on a new genome modification method CRISPR/Cas9 system. Passed. Homologous recombination repair effectively achieves different types of changes in multiple genes in the Plasmodium genome, including gene deletion, gene marking and allele replacement. The development of this system has improved the ability of researchers to modify the Plasmodium genome and promoted the study of Plasmodium biology.