动物实验,无毛鼠急性光损伤 z-bio 2021-01-20 332

　　OBJECTIVE: To explore the defense effects of curcumin and tea polyphenols on UVB-induced acute light damage in hairless rats at different times.

　　Method: Take 36 clean hairless mice and randomly divide them into control group and drug group (0 s, 30 s, 60 s, 120 s, 240 s); each group has 2 mice. Apply curcumin or tea polyphenols on the back of hairless rats 30 min before irradiation, at a distance of 15 cm, and irradiate each group with 3 times the minimum erythema (MED) UVB of about 200-540 mJ/cm2, and take the skin tissue , Make paraffin sections and observe with optical microscope.

　　 Results: The skin of the hairless mice in the control group showed varying degrees of scaly, scattered lymphocytes among the collagen, and changes in collagen red staining, arrangement disorder, and homogenization occurred at 120 s and 240 s. In the drug group, the infiltration of inflammatory cells in the dermis of hairless mice was reduced, and the collagen damage was improved.

　　 Conclusion: Topical curcumin and tea polyphenols can protect hairless mice from acute light damage caused by UVB by reducing the infiltration of inflammatory cells and reducing the degree of collagen damage.