Purpose: Establish a simple method to insert into rodents, and use this method to establish various animal models that require intratracheal infusion.
Method: Choose 60 male Kummin mice anesthetized with midazolam and ketamine, put the 22GA arterial puncture cannula through the optical fiber, guide the trachea through the mouth, connect the ventilator and insert the trachea. make sure.
Result: We successfully inserted 60 mice into the tracheal tube through the optical fiber. Seven days after the autopsy, there were no complications such as bleeding from the mucous membrane of the throat, glottic edema, or tracheal stenosis.
Conclusion: The success rate of tracheal intubation under fiber-mediated visualization is high, and the incidence of related complications is low.