动物实验,斑马鱼消化器官发育缺陷突变体 z-bio 2021-01-21 364

　　OBJECTIVE: To forward genetic screening of mutants with developmental defects in the liver, intestine and gallbladder of zebrafish.

　　Methods: ENU mutagenized wild-type zebrafish and carried out classic F2 generation screening. Whole embryo in situ hybridization using lfabp as a probe and BES-H2O2-Ac fluorescent dye were used to detect the phenotypes of zebrafish early embryo liver, intestine and gallbladder, respectively. .

　　Result: 23 zebrafish digestive organ development defect mutant strains derived from 14 F2 families were screened from 128 mutant genomes, and they were divided into 6 categories according to their phenotypes.

　　Conclusion: The developmental regulation mechanisms of zebrafish liver, intestine and gallbladder are similar and different.