动物实验,树鼩鞭毛虫,18S rRNA基因 z-bio 2021-11-23 577

　　Objective: To observe the morphology and genetic analysis of the horse whip.

　　Method: Remove the contents and feces from the roundabout of the wooden shell, filter and centrifuge, and then directly use the iodine solution for microscopic inspection for staining. The total DNA of worms was extracted by conventional methods, and 18SRNA of flagella was amplified by PCR, and sequenced by BLAST. Use homology analysis and MEGA 5.1 to draw a phylogenetic tree.

　　Result: Morphological observation showed that the beard on the tree shell was Tritrichomonas. Sequence analysis showed that the 18SrRNA in the tree whistle sequence had 99% homology with the 18SrRNA reported by Tritrichomonas fetus (AY754332.1).

　　 Conclusion: Trees can be infected with Trichomonas fetus. The results of this study provide a basis for the quality control of tree parasitology.