动物造模,大鼠脊髓背角胶状质神经元超极化激活电流 z-bio 2021-01-22 301

　　Objective: To study the effect of minocycline on the hyperpolarization activation current (Ih) of glial neurons in the posterior horn of the spinal cord in rats.

　　Method: Select 3-5 weeks old male SD rats, make separate spinal cord cross-sections, use whole-cell patch clamp technique to record 1h SG neurons, and record different concentrations of minocycline (1). ? 300μmol/L) perfusion and observation.

　　Result: About 50% of the recorded SG neurons can record Ih, and the current is blocked by the Ih blockers CsCl and ZD7288. Minocycline can reduce Ih amplitude and Ih current density. This effect is reversible and dose-dependent. The effective inhibitory concentration (IC50) is half of 34μmol/L.

　　Conclusion: Minocycline can inhibit Ih of SG neurons in the posterior horn of the spinal cord, thereby reducing the excitability of SG neurons.