动物造模,大鼠肺缺血再灌注损伤 z-bio 2021-10-26 343

　　Objective: To investigate the effect and mechanism of emulsified fluorocarbon combined with linagliptin on lung ischemia-reperfusion injury.

　　 Method: Establish a rat lung ischemia reperfusion model, and randomly combine it with the control group (C group), rigstradin group (T group), emulsified fluorocarbon group (P group) and rigstradin group. Fluorocarbon (TP group), 10 animals in each group, were administered from the tail vein 5 minutes before the blood supply was restored, and the animals were sacrificed 3 hours after the blood supply was restored. Collect lung tissue and measure malondialdehyde (MDA) and myeloperoxidase (MPO). Observe and record the content of superoxide dismutase (SOD), tumor necrosis factor-α (TNF-α), and pathological changes of lung tissue.

　　 Results: The levels of MDA and MPO in the lung tissues of the T, P and TP groups were significantly lower than those of the C group (P0.05); the lung tissues of the C group had obvious edema and red blood cells. Exudation was found in the alveolar space; there was no obvious edema in the lung tissues of the T and P groups, but a small amount of red blood cells and exudation; there was no obvious edema in the lung tissues of the TP group. There is a small amount of exudation; the pathological score of C group is higher than that of T, P and TP group, and T and P group are higher. TP group (P\u003c0.05).

　　 Conclusion: Emulsified fluorocarbons and regidazine can improve the endogenous scavenging ability of oxygen free radicals, inhibit the accumulation of lung neutrophils, and reduce lung ischemia-reperfusion injury.