动物造模,哮喘小鼠 z-bio 2021-01-22 315

　　Objective: To investigate the effect of chloroquine on airway hyperresponsiveness of asthmatic mice and its possible mechanism.

　　Method: Balb/c mice were randomly divided into control group, asthma group, gold chloride treatment group, dexamethasone treatment group and gold chloride + dexamethasone treatment group. Except for the control group, all mice in the other groups were fed OVA. Sensitivity and challenge, the establishment of asthma mouse model, intraperitoneal injection before each challenge, the control group was treated with the same dose of PBS at the same time within 24 hours after the last challenge, the airway of mice was highly reactive The mouse lung function device was detected. The total number and classification number of cells in bronchoalveolar lavage fluid (BALF) were detected, and pathological sections of lung tissue were prepared, and the pathological changes were observed with an optical microscope after HE staining. The levels of cytokines IL-6 and PGF2α in bronchoalveolar lavage fluid were detected by ELISA.

　　Results: Chloramphenicol can significantly reduce the airway hyperresponsiveness of asthmatic mice (4.5±0.4 vs 6.1±0.9 when inhaled 25 mg/mlAch, 4.6±0.5, 50 mg/mlAch when inhaled P\u003c0.05 vs8.2±1.0, P\u003c0.001); significantly reduced the number of inflammatory cells in the bronchoalveolar lavage fluid of asthmatic mice (P\u003c0.01); significantly reduced the pathological score of asthmatic mice (P\ u003c0.01) P\u003c0.05); the level of PGF2α factor in BALF of asthmatic mice can be reduced to a certain extent; it is related to dexamethasone, bronchial and pericronitis (P\u003c0.05), and perianvascular inflammation (P\ u003c0.05) combined use (u003c0.01), can significantly reduce lung histopathology score (P\u003c0.001), and can significantly reduce the IL- of BALF in asthmatic mice. 6 factor levels (P\u003c0.05).

　　Conclusion: Chloramphenicol can inhibit airway hyperresponsiveness in asthmatic mice. The combination of dexamethasone and chloroquine is more effective than a single agent and may be effective for neutrophilic asthma.