动物造模,内质网应激致海马神经元死亡 z-bio 2021-01-22 425

　　Objective: To investigate the role of up-regulation of KA1 receptor in the death of hippocampal neurons caused by endoplasmic reticulum stress.

　　Method: 70 adult male Kunming mice were randomly divided into alginic acid group (KA group), tunicamycin group (TM) 500μg/ml group, TM1000μg/ml group and TM2000μg/ml group, and injected into the hippocampus. In different time periods (1, 2, 3, 4, 5, 8, 12 hours), KA or different concentrations of TM were perfused into the brain. Before perfusion, brains were collected for Bederson sign score, FJB staining and immunohistochemical analysis of whole brain sections.

　　Results: At 3, 4, 5, 8 hours in the KA group and 4, 5 hours in the TM 2000μg/ml group, the Bederson sign score showed that the central nervous system was significantly damaged, and FJB staining showed that hippocampal cell death was in hippocampal neurons. P-eIF2α and P -The expression of eIF2α increased, and immunohistochemistry showed that the expression of KA1 was significantly up-regulated (P\u003c0.05).

　　Conclusion: After intracavitary injection of KA or TM, the results indicate that KA1 is expressed by endoplasmic reticulum stress. In the process of nerve cell apoptosis, the extramembrane receptor KA1 may first receive the apoptosis signal and transmit it to the cell. The dysfunction of the intracellular reticular tissue induces stress in the intracellular reticular tissue and further promotes neuronal apoptosis.