新生大鼠延髓基本节律性呼吸放电 z-bio 2021-01-25 268

　　Objective: To investigate the effect of drinking during pregnancy on the basic rhythmic respiratory discharge activity (RRDA) of the medulla oblongata slices of the offspring of newborn rats.

　　Method: Prepare an isolated medullary brain slice of a newborn rat, which contains the medial area of the posterior facial nucleus (mNRF), hold the hypoglossal nerve root, and perfuse modified Kleb solution (MKS) with an adsorption electrode. Recorded RRDA. Analyze the changes of the medullary brain slice and the ventral hypoglossal nerve root of RRDA. The experiment is divided into 5 groups: control group, 4% alcohol exposure group, 6% alcohol exposure group, 8% alcohol exposure group, to study the influence of different alcohol exposure concentrations during pregnancy on RRDA, and 10% alcohol exposure group.

　　Results: Controlled inspiratory time (TI), respiratory frequency (RF) and discharge integral amplitude (IA) within 50 minutes, there was no statistical difference in the 4% alcohol exposure group, 6% alcohol exposure group and 8% alcohol exposure group (RRDA) . The RRDA rhythm of the 10% alcohol exposure group was irregular. Compared with the control group, the RRDA of the 4%-10% alcohol exposure group gradually decreased with the increase of alcohol concentration, while the TI shortened. The radio frequency drops and the IA weakens.

　　Conclusion: Drinking alcohol during pregnancy can inhibit the hypoglossal nerve root RRDA in the medulla oblongata of the offspring of newborn rats. This may be the basis for inhibiting the respiratory function of offspring due to alcohol exposure during pregnancy.