动物造模,哮喘小鼠,miR-20b z-bio 2021-11-11 389

　　Objective: To investigate the effect of miR-20b on airway inflammation in asthmatic mice.

　　Method: The BALB/c mice were randomly divided into normal control group, asthma group, asthma + miR-20b simulation treatment group and asthma + miR-20b simulation control group. Ovalbumin (OVA) sensitized and stimulated a mouse model of asthma, and the miR-20b mimic and its control were administered via nasal drops. On the 49th day of the experiment, the total number and classification of cells in the bronchoalveolar lavage fluid (BALF) were detected, the lung tissue was stained with HE, and the pathological changes and the concentration of vascular endothelial growth factor (VEGF) were observed. Detection in BALF by ELISA.

　　Results: After treating asthmatic mice with miR-20b mimics, the difference between the total number of BALF cells and the number of neutrophils and eosinophils was significantly reduced (P\u003c0.01), and the thickening of airway mucosa was reduced . The infiltration of inflammatory cells around the lumen and bronchi is reduced. The content of asthma + miR-20b simulated treatment group was significantly lower than the content of VEGF (18.19±3.67 pg/mL) in BALF in the asthma group (28.55±3.42 pg/mL) (P\u003c0.01).

　　Conclusion: miR-20b can inhibit airway inflammation in asthmatic mice.