动物造模,T细胞,实验性小鼠多发性肌炎 z-bio 2021-10-29 272

　　Objective: To explore the therapeutic value and mechanism of CD4 + CD25 + Foxp3 + Treg cells in experimental autoimmune myositis (EAM) mice.

　　Method: The immunomagnetic bead sorting technique selects enough CD4 + CD25 + Foxp3 + Treg cells from the spleen of BALC/c mice for reinjection. Observe the myopathology of EAM mice in intervention group and non-intervention group. Flow cytometry was used to detect the expression of PD-1 and CTLA-4 on the surface of CD4 + CD25 + Foxp3 + Treg cells in the spleen of the two groups of mice, and to detect IL-10 and TGF-β in peripheral blood. Detected by double antibody sandwich ELISA.

　　Result: Compared with the non-intervention group, the muscle pathological inflammatory cell infiltration of the intervention group was significantly reduced, and the peripheral blood IL-10 and TGF-β levels were significantly lower than the non-intervention group. high. (P\u003c0.05), while the expression of PD-1 and CTLA-4 on the surface of spleen CD4 + CD25 + Foxp3 + Treg cells increased significantly (P\u003c0.05).

　　Conclusion: The therapeutic effect of CD4 + CD25 + Foxp3 + Treg cell reinjection on EAM mice is to increase the peripheral blood IL-10 and TGF-β levels and the spleen CD4 + CD25 + Foxp3 + Treg cell surface PD-1 and CTLA expression -4 Start working.