动物造模,慢病毒,血管紧张素Ⅱ z-bio 2021-01-25 403

　　Objective: To investigate the inhibitory effect of angiotensin converting enzyme 2 (ACE2) on the over-regulation of hepatocyte albumin expression induced by angiotensin II (Ang II) and enhancing cell migration.

　　Method: Regularly culture rat hepatocyte BRL-3A cells, stimulate with AngII (10-7 mol/L), and observe albumin and vimentin in hepatocytes at different treatment times (24, 48 hours). Changes in expression and cell migration; construct lentiACE2 lentiviral vector, establish a cell line stably overexpressing ACE2, intervene with Ang II and A779 respectively, and change the expression and migration of corresponding proteins in liver cells. Cellular immunofluorescence and Western blotting are used to detect changes in protein levels within cells. Use Transwell migration experiment to observe the changes of cell migration ability in different treatment groups.

　　Result: The expression of vimentin in the liver cells of the AngII treatment group was significantly increased, the expression of albumin was decreased, and the cell migration ability was significantly enhanced, and it was time-dependent. ACE2 overexpression rat liver cell albumin expression increased significantly, and vimentin protein expression decreased. This effect was blocked by the MAS receptor inhibitor A779. At the same time, the overexpression of ACE2 significantly inhibited the effect of AngII, significantly reduced the synthesis of vimentin, significantly increased the expression level of albumin, and inhibited cell migration.

　　 Conclusion: Overexpression of ACE2 may inhibit the down-regulation of albumin expression induced by AngII and enhance hepatocyte migration.