动物造模,大鼠星形胶质细胞缺氧/复氧损伤 z-bio 2021-01-25 275

　　Objective: To study the protective effect of Src kinase inhibitor PP2 on hypoxia/reoxygenation injury of rat astrocytes.

　　Methods: Normal control group, hypoxia/reoxygenation group (H/R; hypoxia 8 hours/reoxygenation 24 hours), PP2 + hypoxia/reoxygenation group (PP2 + H/R) for experiment, divided into hypoxia / LPP2, add 10μmol before acting for 24 hours). The MTT method is used to detect the viability of each group of astrocytes, the flow cytometer is used to determine the apoptosis rate of each group of astrocytes, and the Western blot method is used to determine Src kinase and Bax. And Bcl- 2 proteins were detected. Expression in each group of astrocytes.

　　Results: MTT test results showed that cell viability decreased after H/R injury, and cell viability increased after PP2 pretreatment (P\u003c0.01); flow cytometry results showed cell apoptosis after H/R injury (P\u003c0). 01); Western blotting increased Src kinase expression after H/R injury, and decreased Src kinase expression after PP2 pretreatment. The ratio of apoptosis-related protein Bax/Bcl-2 increased after H/R injury, while the ratio of Bax/Bcl-2 decreased significantly after PP2 pretreatment (P\u003c0.01).

　　Conclusion: PP2 can protect astrocytes from HR damage. This mechanism may be related to the inhibition of Src kinase protein expression and activation of anti-apoptotic mechanisms.