C57BL/6J 小鼠 z-bio 2021-01-25 525

　　Purpose: To study the effects of 24-hour rapid eye movement sleep (REMS) deprivation on anxiety behavior and hippocampal nitric oxide (NO) levels in adolescent and adult C57BL/6J mice.

　　Method: Using adolescent (3-4 weeks old) and adult (8-12 weeks old) C57BL/6J mice, the experimental animals in each age group were randomly divided into 3 subgroups. There are 15 in each subgroup: the conventional control (NC) group, the large platform (WP) group and the REMS deprivation 24 hours (REMSD) group. The small platform water environment method was used to create a REMS deprivation model to measure the anxiety behavior of experimental animals in the elevated plus maze. After that, the experimental animals were quickly killed at low temperature, the brains were collected to separate the hippocampus, dissolved and homogenized by centrifugation, and then the supernatant was collected. The level of NO was measured with a microplate reader to measure the nerve nitrogen content of the hippocampus. Oxide synthase (nNOS) was determined by Western blotting. Through the above methods, the effects of 24-hour REMS deprivation on the anxiety behavior, hippocampal NO level and nNOS protein expression in adolescent and adult C57BL/6J mice were observed respectively.

　　 Results: (1) In the NC group, WP group and REMSD group, there was no significant difference in the total number of arm entry, the number and time of arm entry, and the number and time of arm entry. Closed arms. The hippocampal NO level in the EMSD group was significantly higher than that in the NC and WP groups (ps\u003c0.01); the hippocampal nNOS protein level in the EMSD group was significantly higher than that in the NC and WP groups. Group (ps\u003c0.01); (2) The total number of arm access of adult REMSD mice was significantly higher than that of the WP and NC groups (ps\u003c0.01); the number and time of mice entering the open arm was significantly higher high. Lower than the WP group (P\u003c0.01) and NC group (P\u003c0.01), the number and time of entering the closed arm are lower than the WP group (P\u003c0.01) and NC group (P\u003c0.01) . Significantly greater than 01). (U003c0.05); In the NC, WP and REMSD groups, the levels of NO and nNOS protein in the hippocampus of adult mice were not significantly different.

　　 Conclusion: REMSD 24h has different effects on the anxiety behavior of adolescent and adult C57BL/6J mice. This may be related to the difference in hippocampal NO levels and nNOS protein expression changes caused by sleep deprivation.