动物造模,妊娠期高血压大鼠 z-bio 2021-10-29 387

　　Objective: To preliminarily explore the regulatory mechanism of heme oxygenase inducer hemin (hemin) on hypertensive pregnancy (HDCP) rats.

　　 Method: On the 12th day of pregnancy, 18 pregnant SD rats were randomly divided into 3 groups (6 rats/group): HDCP model group, heme intervention group and normal pregnancy group. The HDCP model group and the heme intervention group received nitroso-L-arginine methyl ester (80 mg/kg) for 7 consecutive days from the 14th day of pregnancy to establish the HDCP model. The normal pregnancy group was given the same amount of normal value. Saline intragastric treatment and hemoglobin intervention started from the 16th day of pregnancy, and the group received intraperitoneal injections of hemoglobin (30 mg/kg) daily. The heme oxygenase (HO) activity and carboxyhemoglobin (COHb) levels in placental tissues of each group were measured by spectrophotometry, and soluble vascular endothelial growth factor receptor 1 (sFIt) was measured in the supernatant of placental tissues of each group . Determined by ELSIA. -1) The level of vascular endothelial growth factor (VEGF).

　　 Results: On the 20th day of pregnancy, the blood pressure and 24-hour urine protein of pregnant rats in the HDCP model group were significantly higher than the normal pregnancy group and the heme intervention group (P\u003c0.05), but they had HO activity. The COHb content of the normal pregnancy group and the heme intervention group was significantly reduced (P\u003c0.05), while the blood pressure and 24-hour urine protein of the heme intervention group were higher than those of the normal group (P\u003c0).05). ), HO activity and COHb content were lower than the normal group (P\u003c0.05), but the sFIt-1 level in the placenta tissue of pregnant rats in the HDCP model group was significantly higher than that in the normal pregnancy group and heme. The level of VEGF in the intervention group and placenta tissue (P\u003c0.05) was significantly lower than the normal pregnancy and heme intervention group (P\u003c0.05), but the level of sFIt-1 in the heme intervention group was higher than that of normal pregnancy and heme Vegetarian intervention group. Normal group, very high. The level (P\u003c0.05) and VEGF level were lower than the normal group level (P\u003c0.05).

　　 Conclusion: Heme can reduce blood pressure and urine protein in pregnant women with pregnancy-induced hypertension. The possible mechanism is the up-regulation of HO activity in placental tissue, the increase of metabolite CO, and the decrease and increase of sFIt-1 in placental tissue. Play a role in regulating the level of VEGF.