动物造模,小鼠淋巴细胞 z-bio 2021-01-25 335

　　Objective: To investigate the regulatory effect of human interferon-α (IFN-α) gene transformation on the immune function of mice after oral administration of bifidobacteria.

　　Method: construct the Escherichia coli-Bifidobacterium shuttle expression vector pBADX-hIFNα2b containing human IFN-α gene, electrotransform the IFN-α gene into bifidobacterium, and collect L-induced hIFN-bacteria after expressing α2b. Prepare arabinose as a viable suspension and administer Balb/C mice intragastrically. Experimental group (IFN) mice received 0.2% L-arabinose-induced bifidobacteria-transformed hIFN-α2b expression, 0.1 ml 1010/ml feasible forced feeding, equal dose of negative control group (NC), using empty vector pBADX Transformation of live bifidobacteria by intragastric administration. The blank control group (BC) received the same dose of normal saline in the stomach. Flow cytometry is used to detect the lymphocyte subsets and proportions in mouse thymus, spleen and blood, and flow cytometry kits are used to determine the content of immune factors (such as IFN-γ and IL-4).

　　Result: The proportion of CD3 + CD8 + and CD4 + CD8 + cells in the mouse thymus of the IFN group was significantly increased compared with the NS group and the BC group (P0.05).

　　 Conclusion: Oral oral hIFN-α transformed Bifidobacterium can promote the proliferation and maturation of mouse thymus and spleen lymphocytes, and increase the blood Th1 cytokine level.