动物造模,延缓心肌纤维化 z-bio 2021-01-25 302

　　Objective: To explore the effect of edaravone on reducing oxidative stress and anti-myocardial fibrosis.

　　 Method: 50 8-week-old male SD rats were randomly divided into 5 groups (control group, model group, edaravone low, medium, and high concentration groups). Isoproterenol (ISO) was used to construct a rat myocardial fibrosis model, and each dose group of edaravone (Eda) was simultaneously treated with edaravone for 14 days. On day 15, the degree of myocardial fibrosis, left ventricular mass index (LVMI), collagen volume fraction (CVF) and myocardial tissue type I and III collagen (Col I, Col III), hydroxyproline (Hyp), ultra Oxide dismutase content (SOD) immunofluorescence and Western blot were used to detect the levels of malondialdehyde (MDA) and nitric oxide (NO) in myocardial tissue to detect the expression of TGF-β1 in myocardial tissue.

　　Result: The CVF and LVMI of the model group were significantly higher than those of the control group (both P is 0.000). As the treatment concentration of edaravon group increased (low, medium, high), CVF and LVMI tended to decrease (P\u003c0.05); Col I, Col III and Hyp in the model group were significantly higher than those in the control group (P=0.000). Col I, Col III and Hyp tended to decrease with the increase of the treatment concentration; MDA of the model group was significantly higher than that of the control group (P = 0.000), and the levels of SOD and NO were higher than that of the control group. Both P are 0.000). The SOD and NO of low, medium and high concentrations of edaraben were significantly higher than those of the model group (P\u003c0.05); the TGF-β1 of the model group was significantly higher than that of the control group (P = 0.000), and the levels were low and medium TGF-β1 in high concentration edaraben was significantly lower than that in the model group; MDA was positively correlated with LVMI, CVF, Col I, Col III and Hyp, while SOD and NO were both related to LVMI and CVF, Col I, Col III , Hyp is negatively correlated; TGF-β1 is positively correlated with LVMI, and negatively correlated with CVF, Col I, Col III, Hyp, MDA and SOD, NO.

　　 Conclusion: Edaravone can reduce oxidative stress and inhibit TGF-β1 expression, thereby delaying myocardial fibrosis.