动物造模,阿尔茨海默氏病 z-bio 2021-01-25 341

　　OBJECTIVE: To investigate the characteristics of pathological changes in brain tissue of amyloid cerebrovascular disease based on the establishment of an experimental rat model of Alzheimer's disease.

　　OBJECTIVE: To investigate the characteristics of pathological changes in brain tissue of amyloid cerebrovascular disease based on the establishment of an experimental rat model of Alzheimer's disease. Methods: Under the guidance of a stereotaxic instrument, Aβ1-42 fibers were injected into the hippocampus of the rat to establish an animal model of Alzheimer's disease. The water maze test was used to evaluate the advanced cognitive function of the rat, and HE staining and Congo red were used. Staining and double-labeled immunofluorescence staining with α-actin and Aβ were used to observe the dynamic changes of amyloid deposits in the rat brain and amyloid cerebrovascular disease.

　　 Results: Compared with the sham operation control group, the model group has significant differences in the time of escape latency and the number of platform explorations. HE staining showed that the rat hippocampus in the model group had decreased granular cells, cell degeneration, glial cell proliferation, and degeneration of small blood vessel walls in the hippocampus. Congo red staining showed that Aβ fibers gradually deposited on the wall of small blood vessels, the wall was thickened, and small blood vessels were narrowed or occluded; immunofluorescence staining showed that Aβ fibers in the brain tissue of the model group gradually migrated to small arteries.

　　 Conclusion: Amyloid cerebrovascular disease is the main pathological change of Alzheimer's disease.Methods: Under the guidance of a stereotaxic instrument, Aβ1-42 fibers were injected into the hippocampus of the rat to establish an animal model of Alzheimer's disease. The water maze test was used to evaluate the advanced cognitive function of the rat, and HE staining and Congo red were used. Staining and double-labeled immunofluorescence staining with α-actin and Aβ were used to observe the dynamic changes of amyloid deposits in the rat brain and amyloid cerebrovascular disease.

　　Results: Compared with the sham operation control group, the model group has significant differences in the time of escape latency and the number of platform explorations. HE staining showed that the rat hippocampus in the model group had decreased granular cells, cell degeneration, glial cell proliferation, and degeneration of small blood vessel walls in the hippocampus. Congo red staining showed that Aβ fibers gradually deposited on the wall of small blood vessels, the wall was thickened, and small blood vessels were narrowed or occluded; immunofluorescence staining showed that Aβ fibers in the brain tissue of the model group gradually migrated to small arteries.

　　Conclusion: Amyloid cerebrovascular disease is the main pathological change of Alzheimer's disease.