动物造模,大鼠脂肪肝供肝移植术后 z-bio 2021-11-29 381

　　Objective: To investigate the effect of LXRα RNA interference (RNAi) on the improvement of liver function after liver transplantation in fatty liver donor rats and its mechanism.

　　Method: Fifty SD rats were fed a high-fat diet and 56% alcohol to induce fatty liver. As a liver transplant donor, the average fat change exceeded 60%. 25 fatty liver donor rats in the experimental group were injected with 7×107 TULXRα-RNAi lentiviral suspension (LXRα-RNAi-LV) from the portal vein 72 hours before transplantation. It was injected into 25 fatty liver donor rats in the control group 72 hours before transplantation. A negative control wrench virus (NC-LV) carrier solution was injected into the portal vein. All recipient rats received orthotopic liver transplantation. The levels of liver enzymes, tissue sections, TUNEL, cytokines, tissue proteins and RT-PCR were detected after operation.

　　Results: Compared with the control group, the LXRα-RNAi-LV treatment group significantly inhibited the accumulation of fatty acids in the transplanted liver cells, and significantly increased the levels of liver enzymes and liver tissue damage-related cytokines, and reduced tissue damage Lighter and bigger. The average survival rate of mice increases. T-PCR showed that the level of LXRαmRNA in the experimental group was significantly lower than that in the control group. Western blot showed that the expression of LXRα, SREBP-1c and CD36 was significantly lower than that of the control group.

　　Conclusion: LXRα-RNAi-LV gene therapy can significantly reduce LXRα gene expression in fatty liver donor rats, improve liver function, and increase the survival rate after liver transplantation.