动物造模,小鼠肠道菌群 z-bio 2021-01-25 469

　　Objective: To investigate the effect of dexamethasone pollution in the water environment on the intestinal flora of mice.

　　Method: 20 Balb/c mice were randomly divided into a control group and an experimental group, 5 in each group. The drinking water for the low-dose experimental group contained 0.035g dexamethasone sodium phosphate, the medium-dose group was 0.225g, and the high-dose group was 2.25g. The control group was given drinking water without dexamethasone sodium phosphate. Observe the daily changes of mouse behavior, fur, feces, etc. On the 36th day of perfusion, the mice were sacrificed, and blind tissues were collected to extract bacterial genomic DNA, amplify the variable region of 16SrDNAV6, and analyze the amplified products after denaturing gradient electrophoresis (DGGE). The main bands of the DGGE map were excised, amplified and purified, cloned and sequenced, and their sequences were compared and analyzed by BLAST.

　　Result: The mice in each experimental group showed hypersensitivity, fighting and biting their tails. The cluster analysis of the DGGE chart shows that the flora of each group of mice is relatively stable; the principal component analysis shows that the flora of each group of mice is different; in the analysis of flora diversity, the bacterial species of the control group and the low-dose group The number and the number increased significantly (P\u003c0.01); the type and number of the medium and high-dose groups increased significantly (P\u003c0.01). Sequence analysis of the 16SrDNAV6 region showed that the experimental group and the control group had 15 common bacterial genera and 2 different bacterial genera, and their main bacterial species and proportions had changed. Bacteria of the genus Lactobacillus were established in the control group. Bacteria of the genus Lactobacillus disappeared in the medium-dose and high-dose experimental groups, but bacteria of the genus Shigella appeared.

　　 Conclusion: The pollution of drinking water dexamethasone can affect the nervous system of mice, change the type and quantity of intestinal bacteria in mice, change the proportion of main bacteria, increase the diversity of flora, and inhibit the colonization of intestinal probiotics. Used to invade intestinal pathogens.