腺病毒 z-bio 2021-01-25 371

　　Objective: To synthesize a highly specific shRNA adenovirus vector targeting NF-κB gene and observe whether it directly inhibits the proliferation of cynomolgus monkey endometrial cells cultured in vitro.

　　Method: Design shRNA adenovirus vector with NF-κB-p65 gene and empty gene, culture Chinese mor monkey endometrium cells in vitro, divide them into experimental group and control group, and divide the experimental component into NF-κB-. Transfect p65 shRNA. This group was transfected with adenovirus with empty genes. After co-cultivation, cell proliferation activities such as changes in apoptotic proteins and cell cycle were observed.

　　Result: The PCR and sequencing results of the adenovirus vector are consistent with the expected results, and the virus titer produced by packaging is 1.58×1011U/ml. After F-κB-p65-shRNA adenovirus infects endometrial cells of cynomolgus monkeys, the expression level of apoptosis protein in endometrial cells of the experimental group was significantly higher than that of the negative control group (P). u003c0.05. The comparison between the experimental group and the control group increased significantly, and the number of cells entering the cell division phase was significantly less than that of the control group (P\u003c0.05).

　　Conclusion: The adenovirus carrying the NF-κB gene can accelerate the apoptosis of endometrial cells and inhibit the proliferation of endometrial cells after infecting endometrial monkeys in vitro. The gene is found in the uterus of endometrial monkeys.