动物造模 z-bio 2021-01-26 281

　　Purpose: DEHP exposure to the growth and development of mouse placenta, maternal-fetal interface uNK cell count, and in vivo angiogenesis through exposure to bis(2-ethylhexyl) phthalate (DEHP) during pregnancy to observe its effects.

　　Method: From the first day of pregnancy, do various doses of DEHP125, 250, and 500 mg of DEHP 125, 250, 500 mg via the external forced stomach feeding? kg-1? D-1. Collect and weigh the uterus and placenta specimens. . On the 13th day of pregnancy, HE staining and immunohistochemistry were used to observe the growth and development of mouse placenta, and its effect on the number of uNK cells and angiogenesis at the maternal-fetal interface.

　　Result: (1) Compared with the control group, what is the low-dose DEHP125 250 mg? Kg-1? The dose of D-1 has no significant effect on the number of embryo implants (P\→0.05). However, 500 mg in the high-dose group? Kg-1? D-1 DEHP significantly reduced the number of embryo implantation (P\u003c0.05). In addition, DEHP exposure can cause a significant increase in the rate of fetal miscarriage. (2) Compared with the control group (0.0786±0.0143 g), DEHP reduced the weight of the placenta in a dose-dependent manner of 125 and 250. , 500mg? kg-1? D-1 (each: 0.0637±0.0133, 0.0587±0.0176, 0.0524±0.0183g; P\u003c0.01), significantly reduced the total area of the placenta and the area of the placenta laminar cells; (3) DEHP exposure was significant Reduced the number of fetal vessel branches and vessel collapse and closure; (4) Compared with the control group (105.1±14.2)/HP), DEHP exposure inhibited the number of uNK cells in a dose-dependent manner (83.2±10.3, 60.7± respectively) 12.4, 50.4±14.5/HP; P\u003c0.01).

　　 Conclusion: Exposure to DEHP during pregnancy may seriously affect the growth and development of the placenta, possibly by affecting the formation of blood vessels and the number of uNK cells at the maternal-fetal interface.