动物造模,淋巴细胞DNA损伤 z-bio 2021-01-26 297

　　Objective: To study the genetic toxicity of aniline and its repair kinetics.

　　 Method: Single cell gel electrophoresis (SCGE) technology was used to force a single feeding of 100 mg/kg aniline for 3, 8, 16, 24, 32 hours, and the DNA of liver cells and lymphocytes of KM mice were detected.

　　 Results: The results of the SCGE experiment showed that the tail length and tail moment of liver cells gradually increased from 8 hours, and the degree of DNA damage reached the maximum within 16 hours. Compared with the control group, the difference was statistically significant (P0.05); the length of the tail and the moment of the tail began to lymphocytes at 16 hours, gradually increased and reached the maximum at 24 hours, and gradually recovered with DNA damage. Within 32 hours.

　　 Conclusion: Aniline has potential genetic toxicity to liver cells and lymphocytes. The changes of the two DNA damage indicators have an obvious time-effect relationship, indicating that these two cells have effective DNA repair mechanisms.