动物造模,大鼠脑缺血再灌注损伤 z-bio 2022-01-22 501

　　Objective: To explore the protective effect of acupuncture combined with Danhong injection on cerebral ischemia-reperfusion injury in rats.

　　Method: The middle cerebral artery occlusion method (MCAO) was used to establish a rat middle cerebral artery cerebral ischemia-reperfusion injury model. After 2 hours of ischemia, the thread was removed and reperfused, and then the vein was immediately collected. The injection volume of Danhong injection is 2 mL/kg, puncture Baihui and Zusanli once a day for 3 consecutive days. After 72 hours, Zea-Longa's 5-level 4-point system was used to score the neurological function, and the ischemic brain was stained with Bcl-2 and Bax protein by TTC staining, and the area of cerebral infarction was determined by Western blotting. It was detected in the cortex.

　　Results: Compared with the single use model and the Danhong injection group, the neurological deficit score of rats after acupuncture combined with Danhong injection treatment was significantly reduced, the area of cerebral infarction was reduced, the expression of Bcl-2 protein was increased, and the protein of Bax was increased. Increased expression.

　　Conclusion: The protective effect of acupuncture combined with Danhong injection on cerebral ischemia-reperfusion injury in rats is more obvious than that of Danhong injection alone.