动物造模 z-bo 2022-02-03 384

　　[Modeling mechanism] The incidence of prostate cancer model caused by the combination of chemical carcinogen DMAB and long-acting testosterone propionate (TP) is very high. The cancer originates from the dorsal lobe and the middle lobe, and it has homology with cancer.

　　[Modeling method]

　　1.6-week-old F344 male rat, DMAB, TP.

　　2. Give rats a subcutaneous injection of DMAB 50 mg/kg every other week for 20 weeks. At the same time, a silicone tube containing 40 mg of TP was implanted subcutaneously for 60 weeks and replaced every 6 weeks.

　　3. Model testing ① Blood androgen concentration and acid phosphatase activity: used to study the influence of testosterone on prostate cancer and the detection indicators of prostate cancer. (2) Autopsy: Carefully check the metastasis of bones, lungs, liver and other lobes and the development of prostate tumors; (3) Some rats develop malignant fibrous histiocytoma, sometimes leading to death of the rats. Pay attention to the development of tumors in each lobe of the prostate during the dissection; ④ Paraffin embedding and pathological examination of each lobe suspect the prostate and other organs to have lesions.

　　[Characteristics of the model] The cancer originated from the dorsal lobe to the middle lobe. It has homology with human prostate cancer, has characteristics such as blood lymph node metastasis and elevated serum acid phosphatase, and is similar to people with similar characteristics, so it is considered to be Ideal to guide the animal model. 

      [Model Evaluation and Application] The primary rat hormone prostate cancer tissue was implanted subcutaneously into the back of nude mice. An animal model of transplanted prostate cancer has been established. The established transplanted tumor grows rapidly and has a high potential for metastasis, and is particularly prone to lung metastasis. Tumor has a short lifespan and is easy to observe. It can be used to study the invasion, metastasis and hormone dependence of prostate cancer.