动物造模,前列腺癌细胞 z-bio 2021-01-27 272

　　Objective: To investigate the effect of solanine on the growth of prostate cancer cell Du145 transplanted in nude mice and its molecular mechanism.

　　Method: The highly malignant metastatic prostate cancer cell line Du145 was used as an animal model for in vivo experiments, and nude mice were subcutaneously inoculated with Du145 cells to establish a nude mouse subcutaneous tumor model. After 1 week, the inoculated nude mice were randomly divided into two groups: the solanine experimental group and the normal saline blank control group. 0.2 mL of solanine (50 μg/mL) and normal saline were injected into the middle part of the solid tumor every 3 days. Observe the tumor growth in nude mice. After 3 weeks, the nude mice were killed by cervical dislocation, the tumor tissue was stripped, the tumor weight was measured, and the tumor inhibition rate was calculated based on the tumor weight. Real-time fluorescent quantitative PCR and Western blotting were used to detect the expression of mRNA and protein of cell cycle-related genes in nude mice tumors. Tunel detected tumor tissue death in nude mice in situ in each group.

　　Result: The tumor formation rate of nude mice in the solanine treatment group was significantly decreased, and the tumor growth rate of nude mice was significantly lower than that in the control group (P<0.01). Solanine can inhibit the expression of CyclinD1, CyclinE1, CDK2, CDK4 and CDK6 gene mRNA and protein in tumor cells, and significantly increase the expression of p21 gene mRNA and protein. After Solanine intervention, the tumor tissue apoptosis of nude mice increased significantly ( P<0.01).

　　 Conclusion: Solanine can promote tissue cell apoptosis and inhibit the growth of prostate cancer cell transplanted tumors in nude mice by regulating the cell cycle G1/S checkpoint.