动物造模 z-bio 2021-01-27 328

　　Objective: To study the effect of continuous long-term rapid eye movement sleep deprivation for 5 consecutive days on depression-like behavior and amygdala monoamine oxidase A level in C57BL/6J mice.

　　Method: Adult male C57BL/6J mice were randomly divided into a blank group, a control group and a REM sleep deprivation group, and the rapid eye movement sleep deprivation model was established by the multi-platform water environment method. After sleep deprivation, the field test was used to test the sports performance of each group at different times, and the forced swimming and sugar water preference test were used to test the depression-like behavior of each group. Then the brain was collected to isolate the amygdala, and the expression level of monoamine oxidase A in each group was detected by Western blot and real-time PCR.

　　Result: Within 1-3 days after REM sleep deprivation, the exercise capacity of sleep-deprived mice decreased significantly and returned to normal on the 4th day. The forced swimming experiment on days 7-14 showed that the rats did not move. It takes longer; the preference rate for sugar water is also greatly reduced. Western blot and real-time fluorescent quantitative PCR showed that the expression level of monoamine oxidase A in the amygdala increased significantly.

　　 Conclusion: Continuous sleep deprivation, long-term rapid eye movements can cause behavioral delays, such as depression in mice. This phenomenon may be related to the changes in the level of Amidala monoamine oxidase.