动物造模,兔膝骨关节炎 z-bio 2021-10-14 298

　　OBJECTIVE: To observe the effect of short spines on the Y chromosome sex determining region (Sox9), vascular endothelial growth factor (VEGF) and type X collagen (Col X) expression in articular chondrocytes of rabbit knee osteoarthritis (KOA). The short-term mechanism is used Treatment of spine KOA.

　　Method: 40 New Zealand white rabbits were randomly divided into a normal group (N group), 10 white rabbits and 30 models, the model model is Hulth, the knee osteoarthritis model and the X-ray evaluation model are copied, and the operation is performed. . The modeling was successful, and they were randomly divided into control group (M group), short acupuncture group (CN group), normal acupuncture group (NTN group), treatment started 6 weeks after operation, and untreated group M. In the CN and NTN groups, unilateral Zusanli, medial and lateral eyes of the knee, Liangqiu of the left knee joint and Yinlingquan were taken before and after treatment, and the needles were dried randomly for 20 minutes, once a day for 5 consecutive days, 4 days later. Observe the behavioral changes of each group of New Zealand rabbits; observe the cartilage macromorphology and histological score after the experiment; Westem-Blotting to detect the expression of Sox9 and ColX protein; immunohistochemistry to detect the expression of VEGF and Col X protein. Sox9 was detected by RT-PCR and VEGF mRNA expression.

　　Results: The behavior and cartilage observation of rabbits in the M, CN and NTN groups changed significantly before and after treatment; HE staining can reduce cartilage tissue damage in the CN and NTN groups and tend to be repaired; The cartilage between M and M is between the human scores There is a difference between CN group and CN and NTN group is statistically significant (P\u003c0.01). Compared with M group and VEFG protein, Sox9 and mRNA expression increased, mRNA expression between CN decreased, and Col X protein expression decreased. The treatment group was compared with the NTN group (P\u003c0.01); the difference between the CN group and the NTN group was statistically significant (P\u003c0.01).

　　Conclusion: Short acupuncture can effectively treat KOA, and its mechanism of action is to increase the content of Sox9 in chondrocytes, reduce the expression of VEGF and ColX, and inhibit the differentiation of mast cells to make them normal. Good phenotype. Repair of chondrocytes and cartilage.