动物造模,大鼠肠缺血再灌注损伤,干细胞移植 z-bio 2021-01-28 275

　　Objective: To investigate the effect of glutamine combined with cord blood mesenchymal stem cell (MSC) transplantation in rats with intestinal ischemia-reperfusion injury.

　　Method: The umbilical cord blood mesenchymal stem cells are cultured in vitro and cultured in vitro before transplantation, and the position of the umbilical cord blood mesenchymal stem cells is observed after CM-DiI fluorescent labeling. 80 SD rats were randomly divided into normal control group, ischemia-reperfusion injury group, glutamine group, MSC transplantation group and a combination group of 15 rats in each group. The control group received normal saline enema, the injured group received TNB (S ethanol dilution) enema, and one hour after TNBS modeling, the tail vein of the glutamine group and the MSC transplantation group received 0.45 g/kg glutamine. 1x tail vein. Combine the 1010/L cord blood mesenchymal stem cell suspension, and receive 0.45 g/kg glutamine + cord blood mesenchymal stem cell suspension 1×10 10/L in the tail vein. ELISA method was used to detect intestinal fatty acid binding protein (IFABP), interleukin-6 (IL-6) and superoxide dismutase (SOD) levels in the serum of rats in each group; intestinal tissues of each group were tested once for 3 hours Water content after reperfusion: After glutamine combined with MSC transplantation, RT-PCR expression of caspase-3, NF-kB and Bcl-2 mRNA and protein in rat intestinal mucosal epithelial cells was observed by Western blot.

　　Result: The transplanted MSC cells were observed to be distributed between the intestinal mucosal lymphoid tissue and glandular epithelial cells through fluorescence tracking, and MSC may be involved in the repair process of intestinal ischemia-reperfusion injury, indicating a sexual relationship. Comparison of changes in serum SOD, IFABP and IL-6 levels of rats in each group The serum IFABP and IL-6 levels in the injury group were significantly higher than those in the control group, while the glutamine group and MSC were both at normal levels. The serum SOD content of the injury group was significantly lower than that of the control group, while the glutamine group, MSC transplantation group and combination group were significantly higher than the control group. Combined group. Obviously (P0.05). Compared with the control group, the mRNA and protein expressions of Caspase-3 and NF-kB in the injured intestinal mucosal epithelial cells were significantly up-regulated, while the mRNA and protein expressions of Bcl-2 were significantly down-regulated (P0.05), but both Compare the group with the combination group. The difference is very important (P\u003c0.05).

　　 Conclusion: After glutamine group and MSC transplantation, the degree of intestinal ischemia-reperfusion injury in rats was significantly reduced.