动物造模,-脊髓灰质炎 z-bio 2021-01-28 299

　　Objective: To explore the pathological evaluation method of monkey neurotoxicity in the brain of polio vaccine.

　　Method: Rhesus monkeys were injected into the thalamus by bilateral injection. After 21 days of observation, it was dissected. The brain and spinal cord have been completely removed. After trimming, it is dehydrated and embedded. The sections were stained with HE, observed with gallium blue and an optical microscope, scored the lesion in the nerve tissue, and determined the severity of the lesion according to the score. In this study, 240 animals participated in 12 batches of experiments.

　　Result: This method is used to extract materials from different parts of the left and right brains, such as seams, midbrain, cerebellum, pons, medulla oblongata, cervical enlargement, lumbar enlargement and pathological half-sections. Spinal cord neurotoxicity test, assessment and scoring of bilateral brainstem, cervical and lumbar spinal cord injuries, and determine the scope of these three parts of the disease based on the average score and specific disease conditions. The incidence of certain neurological diseases in this study was 15%. The incidence of various vaccine lesions is similar, with the highest proportion of mild lesions. More serious lesions are mainly found in type III vaccines.

　　 Conclusion: The neurotoxicity test uses a half-section scoring method that objectively reflects the neurological disease to score the disease. The method is simple, easy to implement, and highly practical, which contributes to unification. The standard can be used as a reference for the pathological evaluation method of the polio vaccine neurotoxicity test.