动物造模 z-bio 2021-11-16 321

　　Objective: To study the effect of low-selenium diet on liver and kidney of rats and the effect of macrophage polarization on M1 and M2 in liver and kidney fibrosis.

　　Method: Feed rats with low-selenium and regular diet to establish low-selenium animal model. According to the random number table, 12 female SD rats and 12 male SD rats were divided into two groups: control group (group Z, feed selenium 0.18 mg/kg), low-selenium group (group S, feed selenium 0.02 mg)/ kg) and 109 on the 4th day after feeding, the rats were killed, the pathological changes of liver and kidney were observed by HE staining, and the number of CCR7, CD206, CD163 positive cells in liver and kidney tissues was observed by SP immunohistochemistry. Results: (1) The degree of liver and kidney fibrosis in the low-selenium group was more severe than that of the control group; (2) The staining intensity of liver CCR7 and CD206 in the female and male low-selenium groups was not statistically different between the two groups. Female, male, female, male, P\→0.05). CD163 staining intensity is lower than the control group (both female and male P0.05), and slightly lower than the distal circumflex tubule control group (female P\→0.05, male P0.05, male P0.05), female has statistical significance , There is no statistical significance for men.

　　 Conclusion: The liver and kidney of rats in the low-selenium group have obvious fibrosis. Low-selenium may inhibit the polarization of macrophages into M2 type.