动物造模 z-bio 2021-11-16 309

　　Objective: To explore the effects of benzene on liver and kidney function and blood nucleotide levels in Wistar rats.

　　Methods: Wistar rats were given different concentrations of benzene for 21 days, the levels of different components in the blood were detected by an automatic biochemical analyzer, and the levels of cyclic adenosine monophosphate (cAMP) and cyclic adenosine monophosphate (cAMP) in the blood were detected by enzyme-linked immunosorbent assay (ELISA). The levels of guanosine phosphate (cGMP) and heat shock protein-70 (HSP-70).

　　Results: Wistar rats were given different benzene doses of 0.19, 0.38, 0.76 g/(kg·bw) for 21 consecutive days. The total bilirubin level in the animal’s plasma increased by 134.40%, 173.63%, and 254.75% compared with the control group. Transaminase increased by 70.76%, 85.44%, 106.61%, creatinine increased by 24.54%, 67.46%, 84.50%, creatine kinase increased by 151.35%, 180.85%, 245.54%, urea [0.38, 0.76 g/(kg · Bw) group] increased by 0.48%, 23.43%; alanine transferase level exceeded the upper limit of detection value, both 1185.60%; cAMP level increased by 41.84%, 264.02%, 314.23% compared with the control group, cGMP increased Increased by 31.29%, 40.46%, 272.14%, HSP-70 increased by 82.11%, 187.37%, 1484.21%; the level of amylase decreased by 62.03%, 63.66%, 64.45%, respectively, compared with the control group.

　　Conclusion: Benzene pollution causes changes in the ratio of cAMP and cGMP in the blood, causing metabolic disorders, leading to liver and kidney damage or disease.