动物造模,癫痫 z-bio 2021-11-06 285

　　OBJECTIVE: To preliminarily study the effect of intermittent hypoxic preconditioning (IHP) on epileptic seizures induced by lithium capillin (Li-pilo) in rats and its brain protection mechanism.

　　Method: 96 clean SD rats were randomly divided into a control group, an epilepsy group and 4 intermittent hypoxia preconditioning + epilepsy groups. Rats in the epilepsy group and 4 intermittent hypoxic preconditioning + epilepsy groups (1, 3, 7 and 14 days after 5dIHP preconditioning) were injected intraperitoneally with lithium chloride and pyrrolidine to establish epilepsy models. Subsequently, 240 minutes of seizure behavior, generalized epilepsy latency and quantitative percentage analysis were performed to test the cognitive function of rats through the water maze experiment. Next, use TUNEL labeling and Western blotting to detect the apoptosis of rat hippocampal neurons and related proteins (BCL-2, Bax, and truncated caspase-3).

　　Result: The modified Racine seizure score reached a peak 50-150 minutes after the injection of lithium pyrrole carchloride. After 3 days of intermittent hypoxic preconditioning, the average modified Racine score of the rats in the epilepsy-induced epilepsy group was significantly lower than that of the other groups. The incubation period and incidence of generalized seizures in this group are also significantly different from those in the epilepsy group. (P\u003c0.05).

　　 Conclusion: IHP pretreatment can reduce the sensitivity of rats to epilepsy and protect the brain by inhibiting abnormal cell apoptosis.