动物造模 z-bio 2021-10-26 317

　　Objective: To explore the route and method of hepatic artery cannulation in experimental rabbits.

　　Methods: Thirty New Zealand white rabbits were randomly divided into 5 groups: peripheral intravenous injection, hepatic artery infusion, hepatic artery iodized oil embolization, hepatic artery gelatin sponge particle embolization, and liver direct puncture injection. Each rabbit was given a clinical equivalent dose Of elemene injection. The hepatic artery cannula adopts the Seldinger operation technique. According to the odd and even numbers of the experimental rabbits, the ear artery or the femoral artery are punctured respectively. If one approach fails, the other puncture approach can be switched. Record the operation time, success rate and complications of the puncture catheter and hepatic artery catheterization. Multi-period venous blood was drawn from all experimental rabbits after administration for pharmacokinetic analysis.

　　Results: The success rates of puncturing rabbit ear artery and femoral artery were 55.6% (5/9, 2 unilateral, 3 both sides) and 88.9% (16/18, 12 unilateral, 4 both sides). The success rate of hepatic artery intubation through rabbit ear artery and femoral artery was 0% (0/5) and 100% (16/16), respectively, and the operation time was 28.4±13.6 and 33.9±19.6 min, respectively. Except for the direct liver puncture group and femoral artery puncture group, 1 rabbit died accidentally due to anesthesia and 1 rabbit was euthanized after the failure of arterial puncture. No other experimental rabbits died. After general anesthesia, the peripheral blood vessels of the experimental rabbits were significantly constricted, and it was difficult to collect blood through the rabbit ear vein in a short period of time. In the experiment, 23 rabbits (23/27) were modified from the jugular vein.

　　Conclusion: The feasibility of hepatic artery intubation via rabbit ear artery is low, and femoral artery intubation is the first choice for interventional physicians; rabbit jugular vein blood sampling has inherent anatomical advantages and higher experimental value.